The model shows the interaction of NKX2.5 homeodomain dimer with ANF-242 DNA. NKX2.5 protein is a member of the NK-2 transcription family that is involved in cardiac development and maturation (1). The homeodomain in NKX2.5 binds to consensus DNA motif of TTAAGTG and interacts with other transcriptional factors to regulate gene expression (1). The model NKX2.5 homeodomain consists of three alpha helices and an N-terminal extension. The alpha 2 and alpha 3 helices adopt a helix-turn-helix motif.
Mutations that occur at conserved amino acids within the NKX2.5 homeodomain significantly influences the stability of the complex and lead to a reduction in DNA binding, protein-protein interaction and transactivation activities (1,2). The model aims to explore and encapsulate the key structural features of NKX2.5 homeodomain that plays a pivotal role in gene regulation.
References: 1. DOI: 10.1021/bi300849c 2. DOI: 10.1093/nar/29.15.3258
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