HKX2.5 is a transcription factor that can bind to the proximal promoter of the atrial natriuretic factor (ANF) gene. It plays an essential role in cardiac formation and function, and its mutations typically cause congenital heart diseases during early development. Two identical HKX2.5 proteins will bind to two DNA binding sites, 5-TGAAGTG-3 on one strand, and 3-GTGAACT-5 on the other strand, separated by 5 nucleotides, without interacting with each other. The protein has an N-terminal and a C-terminal A/P-rich sequence, as well as a homeodomain (HD). HD is involved in nuclear translocation, interaction with other transcription factors, and the ANF promoter sequence. This guided tour will illustrate two HKX2.5 homeodomains interacting with the promoter DNA sequence. One protein model shows the general structure of the HD, while the other protein model shows five detailed sites with annotations. References: doi: 10.1021/bi300849c
Comments